General Cell Based Assays
Eurofins Discoverx PathHunter eXpress EDNRB Total Internalization Assay, 2pl Kit. (93-0874E3CP0M)
The PathHunter EDNRB Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of EDNRB via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter FGFR4-β-Klotho Bioassay Kit, 10pl. (93-1060Y3-00090)
The PathHunter FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF19-induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit Colorimetric Method 1/EA
QuickZyme Hydroxyproline Assay Kit 2 x 96 Assays (includes 2x 96-well plates) The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues in a fast and simple 96-well plate format This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed This assay is the ONLY hydroxyproline-based assay that does not need this laborious step resulting in a fast sensitive and easy assay NOTE This kit does not contain tubes or a protocol for collagen hydrolysis If you need these items please order the QuickZyme Total Collagen Assay Application conditioned media cell extracts tissues tissue extracts Sensitivity 2 4 M (0 3 g/ml) Assay-time 2 hours QuickZyme Hydroxyproline Assay can be paired with QuickZyme Total Protein Assay to allow the expression of data as hydroxyproline quantity per total amount of protein
Eurofins Discoverx PathHunter eXpress EpoR/EpoR Dimerization Assay, 10-Plate Kit. (93-0965E3CP0L)
The PathHunter EpoR/EpoR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. erythropoietin) induced dimerization of EpoR/EpoR receptors. This product uses EFC technology. The cell line included overexpresses EpoR target protein fused to PK alongside EpoR target protein fused to EA. Upon ligand-induced dimerization of EpoR/EpoR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MCHR1 Total Internalization Assay, 2pl Kit. (93-0947E3CP5M)
The PathHunter MCHR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MCHR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MCHR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MCHR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rTrkA-rP75 Functional Assay, 10-Plate Kit. (93-1014E3CP16L)
The PathHunter eXpress rTrkA-rP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rTrkA and rP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR2 Total GPCR Internalization Assay, 2pl Kit. (93-0730E3CP0M)
The PathHunter HCRTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-31 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-31 Assay Kit is a multiplex immunoassay that measures mouse interleukin 31 (IL-31) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrator diluent wash buffer read buffer and a vial of lyophilized calibrator that must be reconstituted before use The assay can quantify mouse IL-31 concentrations between 1 5 and 10 000 pg/mL
Eurofins Discoverx AssayComplete Thawing Reagent T6, 100. (92-4106TR)
AssayComplete Thawing Reagent T6 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress HTR2C U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0289E3CP3L)
The PathHunter HTR2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C and EA-tagged β-Arrestin-2. Activation of HTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Adipogenesis Assay Kit
Cayman’s Adipogenesis Assay provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model, using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis, and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
Meso Scale Discovery R-PLEX Human ALK ASY
The R-PLEX Human ALK Assay QuickPlex 5-PL Kit from Meso Scale Diagnostics is a multiplex immunoassay for measuring ALK in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on the QuickPlex SQ 120 instrument The assay takes about 3 hours and needs 12 5 L of sample with a detection range of 19 to 10 000 pg/mL The kit includes reagents for calibration controls diluent detection antibodies read buffer and plates with capture antibodies
Eurofins Discoverx PathHunter eXpress FPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0314E2CP0M)
The PathHunter FPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. formylpeptides) based activation of FPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FPR1 and EA-tagged β-Arrestin-2. Activation of FPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences GFP-CERTIFIED FLUOFORTE Calcium assay kit (10x96wells)
Enzo Life Sciences’ GFP-CERTIFIED FLUOFORTE Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-CERTIFIED FLUOFORTE dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Echelon Biosciences Research Labs PI(4,5)P2 DiC4 500 ug
Phosphatidylinositol 4,5-bisphosphate diC4 (PI(4,5)P2 diC4) is a synthetic, purified dibutanoyl PI(4,5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions, PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues and is also a substrate for class I phosphoinositide 3-kinase (PI3-K). This item is non returnable