General Cell Based Assays
STEMCELL Technologies Wheat Germ Agglutinin WGA i
Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 Ug
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
Bioassay Systems QuantiChrom™ Calcium Assay Kit. For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism.
For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Eurofins Discoverx AssayComplete Cell Plating 2 Reagent, 500. (93-0563R2B)
AssayComplete Cell Plating 2 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GPR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0335E2CP2L)
The PathHunter GPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of GPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR1 and EA-tagged β-Arrestin-2. Activation of GPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Total Antioxidant Capacity Assay Kit.
Total Antioxidant Capacity Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Eurofins Discoverx PathHunter eXpress IL12RB1/IL12RB2 Dimerization Assay, 2pl Kit. (93-1041E3CP0M)
The PathHunter IL12RB1/IL12RB2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cellular ROS Assay Kit (Red)
Cellular ROS Assay Kit (Red)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter FGFR4-β-Klotho Bioassay Kit, 2pl. (93-1060Y3-00089)
The PathHunter FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF19-induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical Cysteinyl Leukotriene Express ELISA Kit, 480 Well
Cayman’s CysLT Express ELISA Kit is a competitive assay that can be used for quantification of CysLTs in urine, plasma, serum, lavage fluids and aspirates, culture media, and other sample matrices. The assay has a range of 7.8-1,000 pg/ml, an average sensitivity (80% B/B0) of 24 pg/ml, and a lower limit of detection (LLOD) of 6.4 pg/ml. NOTE: This kit measures all CysLTs. For measurement of specific CysLTs, please see Cayman Chemical’s Leukotriene E4 ELISA Kit (Item No. 501060) and Leukotriene C4 ELISA Kit (Item No. 501070).
Indigo Biosciences INDIGO Biosciences Activation Reporter Assay System, Human CHRM1, 96-well
This Human Cholinergic Receptor, Muscarinic 1 (CHRM1) Activation Reporter Assay System is a cell-based assay for screening compounds. It quantifies changes in luciferase activity to measure drug-induced changes in CHRM1 activity, identifying activators of CHRM1 or the coupled Ca+2-calcineurin / NFAT signal transduction pathway.
- Utilizes proprietary human cells engineered to constitutively express the human cholinergic receptor, muscarinic 1 (CHRM1).
- Measures drug-induced changes in CHRM1 activity through luciferase reporter gene expression.
- Designed for screening compounds that activate CHRM1 or the Ca+2-calcineurin / NFAT signal transduction pathway.
- Provided in a convenient 96-well format for high-throughput screening.
- Kit includes reporter cells, media, acetylcholine, detection reagents, and a collagen-coated assay plate.
Eurofins Discoverx PathHunter Human Heregulin beta 1 (ErbB2/ErbB4) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Heregulin beta 1 (ErbB2/ErbB4) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of ErbB2/ErbB4. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CRTH2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0291E2CP2L)
The PathHunter CRTH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin D2) based activation of CRTH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CRTH2 and EA-tagged β-Arrestin-2. Activation of CRTH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.